Anti-PLCB3 Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes phospholipase C beta 3 (PLCB3), a key enzyme in phosphoinositide-mediated signal transduction. PLCB3 catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), linking activated G protein-coupled receptors to intracellular calcium release and protein kinase C activation.

The antibody is supplied as an unconjugated, liquid preparation in a 100 µl vial and is validated for Western blot (WB) and immunohistochemistry (IHC) workflows. It has been confirmed to react with human and rat samples, supporting cross-species studies of G protein signaling, receptor pharmacology, and downstream effector pathways in tissues and cell lines.

Working applications include detection of PLCB3 expression in tissue sections for IHC and immunoblotting of cell or tissue lysates. As a polyclonal reagent, the antibody targets multiple epitopes on PLCB3, supporting robust signal generation in research workflows investigating calcium signaling, neuronal G protein pathways, and PLC isoform distribution across human and rat model systems.