Anti-PLCB1 Antibody

Manufactured by Antibodies.com as a primary antibody for signal-transduction research, this polyclonal reagent is raised in rabbit and targets phospholipase C beta 1 (PLCB1), an enzyme also known as 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-beta-1, PLC-I, and PLC-154. The antibody is supplied at a concentration of 1.0 mg/mL in 50 µl volumes, formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, and is unconjugated for downstream flexibility. Purification by affinity chromatography using immunogen yields a high-purity preparation, and the IgG isotype ensures compatibility with standard anti-rabbit secondary reagents. Verified applications from Antibodies.com include Western blot (WB) and immunohistochemistry (IHC), with reactivity confirmed against human, mouse, and rat samples, making it suitable for cross-species studies of PLCB1 expression in tissues and cell lines. For handling, the vial is shipped at 4 °C; upon arrival, users should aliquot the contents and store at −20 °C, taking care to avoid repeated freeze/thaw cycles that can degrade antibody performance. Together, these specifications make this Rabbit polyclonal PLCB1 reagent a reliable choice for researchers investigating phosphoinositide signaling pathways, G-protein–coupled receptor cascades, and related cellular processes where accurate detection of endogenous PLCB1 is required.