Anti-PLC gamma 1 Antibody

This rabbit polyclonal antibody targets phospholipase C gamma 1, a 150 kDa cytoplasmic signaling enzyme that catalyzes phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis into the second messengers IP3 and DAG. From Antibodies.com, the reagent is supplied as an unconjugated 50 µl vial and has been validated for Western blot and immunohistochemistry on human, mouse, and rat samples. PLCG1 contains an N-terminal PH domain followed by SH2 and SH3 regions and a split catalytic core, and its phosphorylation at Tyr783 is widely tracked as a readout of receptor tyrosine kinase activation downstream of EGFR, PDGFR, and FGFR. Researchers studying calcium mobilization, T-cell receptor signaling, or tumor cell migration rely on this antibody to monitor both total expression levels and pathway activity in lysates or fixed tissue sections. For best results, store the vial at -20 °C in small aliquots, avoid repeated freeze-thaw cycles, and protect from light to preserve antibody integrity over multiple experiments.