Anti-PLAT Antibody

Tissue plasminogen activator (tPA), encoded by the PLAT gene, is a serine protease that catalyzes the conversion of plasminogen to plasmin and sits at the core of the fibrinolytic cascade responsible for clot dissolution. The Anti-PLAT Antibody offered by Antibodies.com is a rabbit-derived, affinity-purified polyclonal reagent of the IgG isotype, raised against this antigen and supplied as a 100 µL liquid aliquot in an unconjugated format that gives researchers freedom to pair it with their preferred secondary detection chemistry.

Validated applications cover Western blot (WB) analysis of cell and tissue lysates and immunohistochemistry (IHC) localization in fixed tissue sections. Reactivity has been confirmed against human and rat samples, supporting studies of thrombotic disease, ischemic stroke, vascular biology, neurobiology, and tumor invasion, where altered tPA expression is a frequent point of interest. PLAT is produced by vascular endothelial cells, by specific neuronal populations, and by a number of tumor cell lines, which makes a cross-reactive polyclonal reagent useful for surveying expression across multiple sample types within the same project.

Because the antibody is polyclonal, it recognizes multiple epitopes on the tPA target, which can help when working with samples where the antigen is partially degraded or carries variable post-translational modifications. For routine use, store the vial refrigerated at 2 to 8 degrees C in its original formulation, aliquot if extended storage is anticipated, and avoid repeated freeze-thaw cycles to preserve binding activity across long-running experiments.