Anti-PLA2G4A Antibody

This rabbit polyclonal reagent from Antibodies.com targets PLA2G4A (cytosolic phospholipase A2 alpha, cPLA2α), the group IVA cytosolic enzyme that cleaves arachidonic acid from the sn-2 position of membrane phospholipids to drive prostaglandin and leukotriene biosynthesis.

Validated for Western blot and immunohistochemistry on human samples, the antibody is supplied unconjugated in a 100 µL volume, allowing flexible pairing with rabbit-compatible secondary reagents for chemiluminescent, chromogenic, or fluorescent detection chemistries.

On Western blots of human cell lysates, the antibody detects a band consistent with the expected ~85 kDa PLA2G4A protein. For immunohistochemistry, it supports localization studies in human tissue to map expression across cell types and disease states where altered arachidonate release is being investigated, including inflammatory signaling, reproductive biology, and tumor microenvironments linked to deregulated eicosanoid pathways.

Researchers working on lipid mediator biology, cyclooxygenase-coupled signaling, and downstream eicosanoid production routinely use this reagent to probe cPLA2α expression, phosphorylation status, and subcellular distribution in primary cells and established human cell lines.