Anti-PLA2G1B Antibody

This rabbit polyclonal antibody from Antibodies.com targets phospholipase A2 group IB (PLA2G1B), a secreted pancreatic lipolytic enzyme that hydrolyzes the sn-2 acyl bond of glycerophospholipids to release free fatty acids and lysophospholipids. PLA2G1B is widely studied in the context of acute pancreatitis, pancreatic acinar cell biology, dietary lipid digestion in the small intestine, and its emerging involvement in tumor progression and metabolic inflammation. Validated applications include Western blot (WB) and immunohistochemistry (IHC), making the reagent suitable for protein expression profiling across both tissue sections and whole-cell lysates.

The antibody is raised in rabbit and detects the unconjugated target in Human, Mouse, and Rat samples, supporting translational work that bridges rodent disease models with human clinical specimens. It is supplied as 100 µl of a 1 mg/ml stock in PBS with 0.1% Sodium Azide and 50% glycerol, a glycerol-containing formulation that limits freeze-thaw damage and supports short-term refrigerated storage. For best results, aliquot the antibody upon first use to minimize repeated freeze-thaw cycles, and protect from light when handling any downstream detection reagents.

As a polyclonal reagent, this product offers broader epitope coverage than monoclonal alternatives, an advantage when probing denatured targets on Western blot membranes or fixed, paraffin-embedded sections during IHC staining. Researchers investigating pancreatic exocrine function, gastrointestinal phospholipid metabolism, or inflammatory lipid-mediator signaling pathways will find this primary antibody a reliable tool for both discovery and validation experiments.