Anti-PKR (phospho-T446) Antibody

Phosphorylation-specific detection of Protein Kinase R (PKR, EIF2AK2) at threonine 446 marks the catalytically active form of this dsRNA-activated kinase. The recombinant rabbit monoclonal reagent from Antibodies.com is supplied in 100 µl of TBS buffer (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative.

PKR autophosphorylates at T446 upon binding viral double-stranded RNA, then propagates the antiviral response by phosphorylating eukaryotic translation initiation factor 2α (eIF2α) to suppress global protein synthesis. Researchers studying viral infection, interferon signaling, integrated stress responses, or PKR's emerging roles in tumor suppression and neurodegeneration use this antibody in Western blot and immunohistochemistry workflows on human samples, where it distinguishes the activated kinase from the inactive pool.

The unconjugated format gives flexibility to pair the antibody with custom HRP, biotin, or fluorophore conjugates depending on the detection platform. Recombinant production supports lot-to-lot consistency, and the phospho-epitope specificity allows tracking of dynamic kinase activation in cell lysates or fixed tissue without cross-reactivity to non-phosphorylated PKR. For short-term use, store refrigerated at 2 to 8 degrees C; aliquot to limit freeze-thaw cycles for longer storage.