Anti-PKR (Ab-446) Antibody

Raised in rabbit, this polyclonal reagent from Antibodies.com specifically recognizes Protein Kinase R (PKR, also known as EIF2AK2) when phosphorylated at threonine 446 (the Ab-446 epitope). The antibody is supplied as an unconjugated antiserum in a 100 µl vial, with no fluorophore or enzyme conjugate attached, leaving it freely adaptable to custom labeling workflows in the lab.

PKR is a serine/threonine kinase central to the cellular antiviral response and to stress-induced translational control, acting through phosphorylation of eukaryotic initiation factor 2 alpha. Threonine 446 lies within the activation loop of the kinase domain, and modification at this residue has been linked to PKR activation following dsRNA binding, viral infection, and broader cellular stress signals. Detection of phospho-PKR (Thr446) therefore serves as a read-out of active signaling in interferon pathway studies, virus-host interaction models, and investigations of integrated stress signaling.

Validated applications for this Antibodies.com reagent include western blot, immunohistochemistry, and immunofluorescence, with demonstrated reactivity against human and rat samples. The polyclonal format allows recognition of multiple epitopes on the target, which can be advantageous when monitoring dynamic phosphorylation events. Storage guidance calls for keeping the vial refrigerated at standard laboratory temperatures appropriate for polyclonal antisera, with aliquoting advised to limit repeated freeze-thaw cycles of the working stock.