Anti-PKM2 Antibody

Offered through Antibodies.com, this rabbit polyclonal reagent is raised against pyruvate kinase M2 (PKM2) and is validated for Western blotting, immunohistochemistry, and immunocytochemistry/immunofluorescence workflows. The immunogen is recombinant human PKM2 protein, and the antibody specifically recognizes the endogenous total PKM2 pool in its native form. After affinity purification against the immunogen, the immunoglobulin is supplied at 1.0 mg/mL in a phosphate-buffered saline formulation (pH 7.4) containing 150 mM sodium chloride, 0.02% sodium azide, and 50% glycerol as a cryoprotectant; long-term storage at –20 °C is recommended, with freeze/thaw cycles avoided to preserve activity. The unconjugated preparation is sold in a 50 µL volume and has been demonstrated to react with Human, Mouse, and Rat samples, making it a versatile choice for cross-species metabolic and cancer-biology studies where M2 isoform expression is of interest. Produced in rabbit and affinity-purified rather than antigen-purified, the polyclonal mixture increases the likelihood of detecting multiple epitopes on the PKM2 target, which is useful when probing lysates or fixed tissue sections where epitope masking can occur. Researchers should note that the reagent is intended for research use only and is not validated for diagnostic or therapeutic applications.