Anti-PKM Antibody - Identical to Abcam (ab196560)

Offered by Antibodies.com, this rabbit polyclonal antibody targets PKM and is validated for Western blot and immunofluorescence applications. It is of the IgG isotype, supplied unconjugated, and raised in a rabbit host, with reactivity to human samples indicated in the catalogue listing.

Manufacturer documentation states the antibody was raised against a recombinant fusion protein corresponding to amino acids 11-221 of human PKM2, with reactivity demonstrated against human, mouse and rat samples. The product is supplied as a liquid in Phosphate Buffered Saline (pH 7.3) with 50% glycerol and 0.02% sodium azide, and has been affinity purified. Reported molecular weight is 62 kDa with a predicted molecular weight of 58 kDa. Recommended dilutions are 1:500-1:2,000 for WB, 1:50-1:200 for ICC/IF, and 1 µg/ml as a starting concentration for ELISA. Two scientific validation images are referenced.

The antibody is shipped at 4°C and should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided.