Anti-PKLR Antibody

Targeting pyruvate kinase L/R (PKLR), this rabbit polyclonal reagent from Antibodies.com supports western blot detection of the glycolytic enzyme across human, mouse, and rat samples. The unconjugated antibody is supplied as a 100 µl vial formulated at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2. Investigators working on hereditary pyruvate kinase deficiency, non-spherocytic hemolytic anemia, erythrocyte energy metabolism, and hepatic carbohydrate regulation rely on this reagent to probe PKLR expression in cell line and tissue lysates.

The polyclonal host response recognizes multiple epitopes on the target protein, supporting robust signal in denaturing SDS-PAGE whole-cell extracts. Researchers are encouraged to empirically optimize primary antibody dilution based on antigen abundance in their particular system. Storage and handling guidance: refrigerate the vial between uses, protect from light to preserve activity, and aliquot the antibody stock to limit freeze-thaw cycles. Sodium azide serves as a microbial preservative, so the reagent should be paired with azide-compatible buffers and is not appropriate for live-cell staining workflows. The product is intended for research use only and is not approved for diagnostic or therapeutic applications.