Anti-PKCth (Ab-676) Antibody

This Rabbit polyclonal antibody to PKCth (Ab-676) from Antibodies.com is supplied as a 50 µl liquid aliquot at 1.0 mg/mL in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol. The unconjugated reagent was raised in rabbit against a synthetic peptide spanning amino acids 674–678 (R-L-S-F-A) of human PKCth (PRKCQ, UniProt Q04759, nPKC-theta) and detects endogenous levels of the total PKCth protein. The polyclonal IgG host antibody is recommended for Western blotting at 1:500–1:1000, immunohistochemistry at 1:50–1:100 and immunofluorescence at 1:100–1:200, with reactivity confirmed on human samples and an observed molecular weight near the predicted 80 kDa. For short-term handling the reagent is stable at 4 °C for up to six months, while long-term storage at −20 °C is recommended with avoidance of repeated freeze/thaw cycles; the product is supplied for research use only and is not intended for diagnostic or therapeutic applications.