Anti-PKC-pan (αT497) Antibody

This rabbit polyclonal antibody from Antibodies.com targets the PKC-pan family and recognizes the conserved phosphorylated threonine in the activation-loop region (βT497) shared by multiple Protein Kinase C isoforms. Raised in rabbit and supplied as an unconjugated reagent at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide (approximately pH 7.2), it is validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human, mouse, and rat samples.

The T497 activation-loop motif is a hallmark of catalytically active PKC, so this reagent serves as a read-out of PKC signaling activation in cell lysates and tissue sections. Researchers commonly deploy it to investigate PKC-dependent pathways linked to receptor-mediated signal transduction, cytoskeletal reorganization, and cell-cycle regulation, and the broad isoform coverage makes it practical for screening activation status across conventional, novel, and atypical PKC family members in parallel without raising separate isoform-specific primaries.

The reagent is supplied as a 100 µl vial and should be stored at -20 °C in working aliquots to limit freeze-thaw cycles; for short-term bench use it can be kept refrigerated at 4 °C. Sodium azide at 0.05% acts as a preservative, and the concentrate can be diluted in PBS-based diluent for blotting or immunostaining protocols, with the unconjugated format allowing direct pairing with a secondary antibody of the researcher's choice for fluorescence or chromogenic detection.