Anti-PKC θ (phospho-S676) Antibody

Rabbit polyclonal reagent from Antibodies.com raised against protein kinase C theta phosphorylated at serine 676. Supplied as 100 µl at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, the unconjugated antibody is recommended for Western blot, immunohistochemistry, and immunofluorescence on human, mouse, and rat samples.

Protein kinase C theta is a calcium-independent, diacylglycerol-dependent member of the novel PKC subfamily that is recruited to the immunological synapse during T cell receptor engagement and is required for NF-κB activation in mature T lymphocytes. Serine 676 lies within the C-terminal turn motif and has been reported as an activation-associated autophosphorylation site, so this antibody is a useful marker for cells in which the kinase has been switched on downstream of antigen receptor stimulation. The polyclonal pool from the rabbit host improves recognition of the phospho-S676 epitope across multiple flanking residues and pairs with standard anti-rabbit IgG secondary reagents.

Aliquot the antibody into working volumes and store at -20 °C to limit freeze-thaw cycles; protect from light. For research use only.