Anti-PKC α (phospho-T505) Antibody

Affinity-purified polyclonal reagent raised in rabbit against Protein Kinase C alpha, specifically recognizing the autophosphorylated activation-loop residue threonine 505. Phosphorylation at T505 is mediated by PDK1 and serves as a marker of catalytic activation, making this antibody a useful tool for tracking downstream signaling events in studies of cell proliferation, apoptosis, and receptor-driven pathways.

The unconjugated IgG is supplied at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide, packaged as 100 µl, and is validated for Western blot and immunohistochemistry on human, mouse, and rat samples. Researchers applying it to tumor biology, vascular smooth muscle signaling, or insulin-response models rely on the phospho-specific recognition to distinguish active kinase from total protein pool.

Storage at -20 °C in working aliquots is recommended to preserve phospho-epitope binding, and the sodium azide acts as a microbial preservative. From Antibodies.com, this Primary Antibody supports clean read-outs of acute PKC alpha activation across cell-based and tissue-based assays.