Anti-PKC α (E653) Antibody

Rabbit polyclonal antibody raised against protein kinase C theta (PKC θ), supplied by Antibodies.com as an unconjugated primary antibody validated for immunohistochemistry (IHC). The epitope corresponds to a region surrounding residue E653 of the human PKC θ sequence, making this reagent useful for studies of T-cell receptor signaling, NF-κB activation, and downstream kinase cascades in lymphoid tissue sections.

Provided at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the antibody is aliquoted at 100 µl per vial and is intended for research use only. The host species is rabbit, the clonality is polyclonal, and the conjugate is unconjugated, allowing direct selection of detection chemistries and anti-rabbit secondary reagents.

Reactivity has been confirmed against human, mouse, and rat samples, supporting translational work across species. The reagent performs on fixed tissue sections using standard antigen retrieval protocols and is compatible with both chromogenic and fluorescence-based detection. For routine use, refrigerate at 4 °C; for longer storage, aliquot and freeze at -20 °C, avoiding repeated freeze-thaw cycles to preserve binding activity. Lot-specific validation data and recommended dilutions are supplied with the vial.