Anti-PIWIL1 Antibody

The PIWIL1 protein, also known as Piwi-like protein 1 or HIWI, belongs to the Argonaute family and is central to piRNA-mediated transposon silencing, germline maintenance, and stem cell self-renewal. This unconjugated IgG polyclonal reagent is raised in rabbit and validated for Western blot (WB) and immunofluorescence (IF) workflows. It detects endogenous PIWIL1 in human, mouse, and rat samples, supporting studies of spermatogenesis, transposon regulation, and the emerging links between PIWIL1 reactivation and oncogenic signaling in solid tumors.

Supplied by Antibodies.com as a 200 µl vial, the antibody can be aliquoted upon arrival to limit freeze-thaw cycles and preserve lot-to-lot consistency across multiple experiments. The unconjugated format allows researchers to couple it to their preferred fluorophore or HRP, supporting both immunofluorescence imaging of germline tissue and Western blot detection of full-length PIWIL1. Storage at -20°C in working aliquots is recommended for long-term stability; avoid repeated freeze-thaw cycles to maintain titer and binding performance.

Common research applications include characterization of PIWI/piRNA pathway components in testis lysates, investigation of transposable element silencing in early embryonic stages, and screening of cancer cell lines where aberrant PIWIL1 expression has been reported. The polyclonal nature of the reagent offers epitope diversity, increasing the likelihood of detecting PIWIL1 across species and isoforms under denaturing (WB) or native (IF) conditions.