Anti-PITX3 Antibody

PITX3 (Paired-like homeodomain transcription factor 3) is selectively expressed in mesencephalic dopaminergic (mDA) neurons as early as embryonic day 11.5 and governs terminal differentiation of the substantia nigra pars compacta (SNc) subtype. This rabbit polyclonal IgG reagent from Antibodies.com, supplied unconjugated in a 200 µl vial, is validated for Western blot detection across human, mouse, and rat samples. Published neuroscience work uses comparable PITX3 antisera at dilutions near 1:1,000 on RIPA lysates separated by SDS-PAGE, with HRP- or IRDye-conjugated goat anti-rabbit IgG secondaries and chemiluminescent or near-infrared readouts on PVDF or nitrocellulose membranes. Recommended handling is to aliquot on first thaw and store frozen at -20 °C, avoiding repeated freeze-thaw cycles so the antibody can be reused across multiple immunoblotting experiments. Common research applications include tracking mDA differentiation in MES23.5 and MN9D dopaminergic cell models, monitoring PITX3 protein levels in ventral midbrain tissue extracts, and pairing PITX3 detection with co-markers such as tyrosine hydroxylase, NURR1, or LMX1B in Parkinson's disease and stem cell differentiation studies. Conditional Pitx3 knockout studies further link the homeodomain protein to age-related mDA neurodegeneration and to congenital anterior segment disorders, making reliable immunological detection broadly informative for both neuroscience and ophthalmology research.