Anti-PIP5K1B Antibody

A rabbit polyclonal antibody raised against phosphatidylinositol-4-phosphate 5-kinase type-1 beta (PIP5K1B), supplied by Antibodies.com as an unconjugated IgG reagent in a 200 µl vial. PIP5K1B is the lipid kinase that converts phosphatidylinositol 4-phosphate to phosphatidylinositol 4,5-bisphosphate (PIP2), a phospholipid central to plasma membrane signaling, cytoskeletal regulation, and synaptic vesicle cycling in neurons and other cell types.

The antibody is validated for Western blot and immunohistochemistry on human samples, supporting research into phosphoinositide metabolism, neuronal function, focal adhesion dynamics, and membrane trafficking. Investigators use this reagent to probe endogenous PIP5K1B expression in cell lysates and fixed tissue sections, where it complements downstream readouts of PIP2 levels and actin organization. Its polyclonal profile is useful for detecting the full-length kinase across denaturing and native conditions, and the unconjugated format supports flexible pairing with HRP-, fluorescent-, or biotin-labeled secondary reagents depending on the chosen detection platform.

For handling, refrigerate the vial on receipt, protect from light, and aliquot before freezing if repeated use is planned; brief centrifugation helps recover the full 200 µl volume, and limiting freeze-thaw cycles preserves affinity over the product's shelf life.