Anti-PIASx Antibody

The Anti-PIASx polyclonal reagent from Antibodies.com is a rabbit-derived primary antibody directed against the PIAS2 protein, a SUMO E3 ligase that modulates transcription through covalent modification of nuclear targets. Provided at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, this unconjugated polyclonal antibody is supplied as a 100 µL vial sized for western blot, immunohistochemistry, and immunofluorescence workflows. The antibody has been validated on human, mouse, and rat samples, supporting cross-species studies of PIASx expression in cultured cells, fixed tissue sections, and whole-cell lysates. PIASx participates in SUMO-dependent regulation of transcription factors including STAT family members, p53, and the androgen receptor, making this reagent useful for investigations of post-translational modification, gene-expression control, and DNA damage response pathways. Researchers can apply it to chromatin fractionation experiments, nuclear body assembly studies, and signaling-pathway analyses where endogenous PIASx abundance is being tracked. Storage at -20 °C is recommended, with aliquoting to limit freeze-thaw cycles, and the glycerol-containing buffer protects the antibody during routine handling at the bench.