Anti-PIAS3 Antibody

This rabbit polyclonal antibody targets PIAS3, a SUMO E3 ligase that modulates STAT3 signalling and broader transcriptional programmes. Raised in rabbit and supplied as an unconjugated polyclonal reagent at 100 µl, it is validated for Western blot and immunohistochemistry workflows on human, mouse, and rat samples.

PIAS3 functions as a post-translational regulator that conjugates SUMO to substrate proteins, influencing nuclear translocation, chromatin association, and protein stability of transcription factors including STAT3 and SMAD family members. Researchers studying cytokine-driven signalling, inflammatory pathways, and cancer biology use anti-PIAS3 reagents to probe receptor-mediated activation cascades and to map SUMOylation patterns in tumour tissue and cell line models.

From Antibodies.com, the reagent is provided as a liquid aliquot intended for repeated use when stored under standard refrigerated conditions and protected from light. Working dilutions support detection of endogenous protein expression across whole-cell lysates, fixed tissue sections, and archival material, while the antibody's cross-species reactivity enables comparative studies in xenograft, knockout, or knockdown experimental designs.

Typical applications include verification of PIAS3 expression in breast carcinoma, glioma, and leukaemia research models, alongside investigation of innate immune regulation, STAT3-driven cytokine signalling, and SUMO-dependent chromatin remodelling. The unconjugated format allows direct pairing with secondary antibodies chosen by the researcher for chromogenic, fluorescent, or chemiluminescent detection platforms in single-use or batch experimental setups.