Anti-PIAS 3 Antibody

Raised in rabbit against a synthetic peptide derived from the N-terminal region of human PIAS 3, this affinity-purified polyclonal reagent from Antibodies.com is supplied as a 50 µl liquid at 1 mg/ml in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The unconjugated antibody detects endogenous levels of the PIAS 3 protein (E3 SUMO-protein ligase / protein inhibitor of activated STAT3) and is validated for Western blot, immunohistochemistry and ELISA workflows in human, mouse and rat samples. Because the host is rabbit and the clonality is polyclonal, the immunoglobulin class is IgG, which means it pairs cleanly with standard goat anti-rabbit IgG H&L secondaries in HRP, FITC, biotin and AP formats. For best stability the vial should be stored at –20 °C, where it remains usable for up to one year, avoiding repeated freeze-thaw cycles. Researchers working on STAT signaling, sumoylation pathways or transcriptional regulation will find this reagent a reliable primary for confirming PIAS 3 expression across cell and tissue lysates, while the small50 µl pack size suits pilot studies, antibody screening panels and labs that want to validate performance before scaling up to the100 µl format.