Anti-PI3K p85α/p55γ Antibody

Phospho-specific polyclonal reagent from Antibodies.com directed against the p85α regulatory subunit of class IA phosphoinositide 3-kinase (PI3K), with cross-recognition of the p55γ isoform. It selectively binds the activated forms carrying a phosphate group at Y467 of p85α and Y199 of p55γ, supporting studies of acute PI3K pathway activation triggered by receptor tyrosine kinases, cytokine receptors, and G-protein-coupled signals.

Supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, this unconjugated rabbit polyclonal antibody is validated for western blot, immunohistochemistry, and immunofluorescence applications on human, mouse, and rat samples. Short-term refrigerated storage is recommended for working aliquots, with the sodium azide acting as a preservative against microbial growth in the vial. Researchers in oncology, metabolic disease modeling, and immune signaling use this reagent to map phosphorylation dynamics of the PI3K/AKT axis, including AKT activation downstream of insulin and growth factor cues.