Anti-PI3K p110γ (L89) Antibody

Affinity-purified rabbit polyclonal reagent raised against the gamma catalytic subunit of class I phosphoinositide 3-kinase. The immunogen corresponds to a region surrounding residue L89 of the human target, encoded by the PIK3CG gene. Supplied as 100 µL of an unconjugated, preservative-stabilized stock at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2, the antibody from Antibodies.com is validated for Western blot and immunohistochemistry on human samples.

PI3K p110γ couples G-protein-coupled receptors to PIP3 generation in leukocytes, where it directs chemokine-driven migration, mast cell degranulation, and the oxidative burst. Researchers studying innate immune cell signaling, models of inflammatory disease, and the tumor microenvironment apply this reagent to probe isoform-specific activity alongside parallel p110α, p110β, and p110δ readouts. The expected 110 kDa band observed by Western blot and the cytoplasmic or membrane-associated staining patterns seen by IHC serve as useful positive indicators of a productive experiment.

For handling, refrigerate the vial in the dark and avoid repeated freeze-thaw cycles by aliquoting the antibody solution on first use; the sodium azide in the formulation also requires careful disposal of downstream waste. Each lot is affinity-purified against the L89 epitope to limit off-target recognition in phosphoinositide signaling-pathway studies, supporting reproducible detection across replicates.