Anti-PHPT1 Antibody

PHPT1 (Phosphohistidine Phosphatase 1) is a ~14 kDa catalytic enzyme that dephosphorylates phosphohistidine residues on proteins and peptides, drawing interest in studies of non-canonical histidine phosphorylation, ion channel regulation, and T-cell receptor signaling. This unconjugated rabbit polyclonal reagent from Antibodies.com was raised against the human PHPT1 sequence and has been validated for western blot, immunohistochemistry on fixed tissue, and immunofluorescence in cultured cells.

The antibody is verified reactive with human, mouse, and rat samples, covering the standard mammalian model systems used in cell signaling and metabolic research. It is supplied as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as cryoprotectant; store at -20°C and keep working aliquots refrigerated to limit freeze-thaw cycling on the antibody stock.

Because PHPT1 is a small ~14 kDa protein, the polyclonal format offers an advantage: recognition of multiple epitopes improves tolerance for partial degradation or stringent denaturation conditions during SDS-PAGE and western blot work. The unconjugated preparation also pairs cleanly with secondary reagents chosen for western blot detection, immunohistochemistry, or immunofluorescence imaging without intrinsic dye or enzyme crosstalk.

Intended for research use only.