Anti-PHO1 Antibody

Antibodies.com offers this rabbit polyclonal reagent directed against the PHO1 target, an endogenous mammalian protein of interest in studies of phosphate metabolism and membrane transport biology. The antibody is supplied as an unconjugated IgG fraction at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, packaged in a 100 µL vial ready for direct dilution into standard immunoblotting workflows. Validated reactivity covers human, mouse, and rat samples, supporting comparative work across cultured cell lines, primary cells, and tissue lysates prepared from each species. Typical western blot protocols load 5-10 µg of total protein per lane on SDS-PAGE, transfer to a suitable membrane, and probe with the antibody at a working dilution in blocking buffer; signal is then developed with standard HRP-conjugated secondary reagents and chemiluminescent substrates. For active use, store refrigerated and protected from light; for longer term, aliquot the vial and freeze to limit freeze-thaw cycles. This polyclonal reagent is supplied for research use only and is well suited to laboratories investigating phosphate homeostasis, membrane transporter regulation, and related signaling pathways in mammalian systems.