Anti-PHF21A Antibody

A rabbit polyclonal antibody from Antibodies.com targets PHD finger protein 21A (PHF21A), a chromatin reader that recognizes unmethylated histone H3 lysine 4 residues and functions as a core subunit of the LSD1-CoREST transcriptional repressor complex. Supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, this 100 µl vial is validated for western blot and immunohistochemistry applications in human and mouse samples. The unconjugated format permits flexible pairing with custom secondary antibodies and detection chemistries, while the polyclonal antibody pool allows recognition of multiple epitopes on the target. Researchers use this reagent to investigate neuronal differentiation pathways, histone modification crosstalk, and the consequences of PHF21A loss in Potocki-Shaffer syndrome and certain solid tumors. For western blot, clear band detection is achievable in whole-cell lysates, and immunohistochemistry reveals nuclear PHF21A staining patterns in formalin-fixed paraffin-embedded tissue sections. Storage at -20°C is appropriate thanks to the included glycerol, and aliquoting upon first use is recommended to minimize freeze-thaw cycles across long-term studies.