Anti-PHC3 Antibody

This polyclonal reagent from Antibodies.com targets PHC3 (Polyhomeotic-like protein 3), a core subunit of the Polycomb Repressive Complex 1 (PRC1) that mediates monoubiquitination of histone H2A at lysine 119 (H2AK119ub) and contributes to long-term transcriptional silencing of developmental loci.

Raised in rabbit, the antibody is supplied as an unconjugated antiserum at 100 µL per vial and is validated in Western blot and immunofluorescence workflows. It recognizes both the human and murine orthologs, supporting cross-species studies in cell line panels, primary cultures, and fixed tissue sections where PRC1-mediated chromatin regulation is being investigated.

Recommended uses include probing PRC1 complex composition in embryonic stem cells, tracking polycomb group occupancy during differentiation, and examining PHC3 expression patterns in cancer research models. For Western blot, aliquot the antibody and store at -20°C; avoid repeated freeze-thaw cycles to preserve titre. For immunofluorescence, the unconjugated format allows pairing with user-selected fluorophore-conjugated secondary reagents.

Researchers studying histone modification pathways, X-inactivation, or polycomb-mediated gene repression will find this reagent valuable for routine validation as well as exploratory chromatin biology experiments.