Anti-PHC3 Antibody

Polyhomeotic Homolog 3 (PHC3) is a core subunit of the Polycomb Repressive Complex 1, contributing to H2A ubiquitination, chromatin compaction, and long-term transcriptional silencing during embryonic development and in adult tissues. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against PHC3 and validated for Western blot, immunohistochemistry, and immunofluorescence workflows in human and mouse samples.

Supplied at 1 mg/ml in PBS with 0.1% Sodium Azide and 50% glycerol as a cryoprotectant, the antibody is provided as a 100 µl vial. Aliquoting on arrival and storage at -20 °C is recommended to limit freeze-thaw cycles and preserve reagent activity over multiple experiments.

Polyclonal reactivity to PHC3 supports cross-species studies in stem cell biology, lineage specification, and investigations of Polycomb-mediated gene repression in cancer cell lines and primary tissue. The unconjugated format offers flexibility to pair the reagent with user-selected secondary antibodies for fluorescent or chromogenic detection, or to use it directly in chromatin immunoprecipitation and co-immunoprecipitation workflows where an unconjugated primary is preferred.