Anti-PHB Antibody

The polyclonal antibody targets Prohibitin (PHB), a conserved ~30 kDa inner-mitochondrial-membrane protein recognized for its roles in mitochondrial respiratory chain organization, cell-cycle control, and apoptosis regulation. Raised in rabbit and affinity-purified, this unconjugated reagent from Antibodies.com is supplied as a 100 µl liquid aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol. The antibody has been validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows on human, mouse, and rat samples, where it recognizes endogenous mitochondrial PHB as a band near 30 kDa. Researchers use it to probe mitochondrial integrity and stress responses in cancer biology, neurodegeneration, and cellular senescence studies, where PHB expression and subcellular localization shift with metabolic load. The glycerol-containing buffer tolerates standard -20 °C freezer storage with minimal freeze-thaw loss of titer, and the 100 µl volume supports multiple rounds of staining and blotting without immediate re-aliquoting.