Anti-PGK1 Antibody

Phosphoglycerate kinase 1 (PGK1) is a key glycolytic enzyme that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, generating ATP in the process. This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against PGK1 and is supplied as 100 µl of polyclonal antiserum validated for Western blot, immunohistochemistry, and immunofluorescence workflows. It cross-reacts with human, mouse, and rat samples, enabling comparative studies across species in metabolism and oncology research. PGK1 sits at a metabolic branch point and has attracted sustained interest in cancer biology because tumor cells frequently upregulate glycolysis; the reagent is therefore commonly applied to evaluate PGK1 expression levels across tumor and normal tissue panels, to probe subcellular localization in fixed cells, and to serve as a loading reference in glycolysis-focused Western blots. For handling, aliquot on receipt to limit freeze-thaw cycles and store refrigerated for short-term use, with long-term storage at -20 °C to preserve antibody activity.