Anti-PFN2 Antibody

This polyclonal antibody from Antibodies.com targets Profilin 2 (PFN2), an actin-monomer-binding protein that regulates cytoskeletal remodeling and synaptic vesicle endocytosis in neurons. Raised in rabbit, the reagent is supplied as an unconjugated preparation in a 100 µl vial, intended for direct use in Western blot and immunohistochemistry workflows. Antibody reactivity has been validated against human, mouse, and rat samples, supporting cross-species studies of neural tissue, glioma cell lines, and models of cytoskeleton-dependent signaling. The polyclonal format increases the likelihood of recognizing multiple epitopes on PFN2, which helps when examining post-translational modifications or splice variants. In Western blot, the antibody detects the endogenous ~15 kDa PFN2 band in lysates from brain, spinal cord, and certain tumor-derived cell lines. For immunohistochemistry, it enables localization of PFN2 in formalin-fixed paraffin-embedded brain sections, where Profilin 2 concentrates in dendritic spines and presynaptic terminals. Researchers studying long-term potentiation, actin dynamics, neuronal differentiation, and PFN2 dysregulation in cancer rely on this reagent for reproducible detection across experimental replicates.