Anti-PFKL Antibody

Produced by Antibodies.com as a rabbit polyclonal reagent, this affinity-purified antibody targets the human phosphofructokinase, liver type (PFKL) enzyme and is supplied in a 100 µl vial at a working dilution suggested around 1:1000 for Western blot applications. The immunoglobulin is unconjugated and of the IgG isotype, raised against a recombinant protein fragment corresponding to amino acids 475–691 of PFKL, and purified by antigen-affinity chromatography to reduce non-specific background. Researchers can rely on it for Western blotting, immunohistochemistry, and immunocytochemistry/immunofluorescence workflows, with reactivity confirmed against human samples including the Hep G2 cell line shown in the validation data. The buffer is a 0.1 M Tris-buffered saline (pH 7.0) containing 10 % glycerol as a cryoprotectant, with 0.01 % thimerosal added as a preservative; the product is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, avoiding repeated freeze/thaw cycles to preserve activity. Designed for research use only and not for diagnostic or therapeutic applications, this Antibodies.com reagent offers a reliable option for laboratories investigating glycolytic metabolism, since PFKL catalyses a rate-limiting step of glycolysis and is frequently studied in the context of cancer cell energetics, liver physiology, and metabolic disease models.