Anti-PELP1 Antibody

PELP1 (Proline-, Glutamate-, and Leucine-Rich Protein 1) is a transcriptional scaffolding coactivator best characterized for its role in modulating estrogen receptor (ERα) signaling, ribosomal biogenesis, and SUMO-dependent transcriptional regulation. Aberrant PELP1 expression has been documented in hormone-driven cancers including breast, ovarian, endometrial, and prostate tumors, where it has been linked to dysregulated cell cycle progression and metastatic behavior, making it a useful marker in oncology and signaling research.

This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the PELP1 target and validated for Western blot (WB) and immunohistochemistry (IHC) workflows. The antibody cross-reacts with human, mouse, and rat samples, supporting translational studies that compare expression across model systems and patient-derived tissue. It is supplied in a single-use vial as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, a formulation that supports routine laboratory storage at -20°C with minimal freeze-thaw cycling.

For IHC, antigen retrieval methods such as citrate buffer at pH 6.0 are commonly applied, and the unconjugated format allows pairing with HRP- or fluorophore-conjugated secondary antibodies of the researcher's choice. Standard WB use generally falls in the 0.5–2 µg/ml range when diluted in blocking buffer, while IHC use typically requires titration between 1–10 µg/ml depending on tissue fixation and detection chemistry.