Anti-PELO Antibody

This unconjugated rabbit polyclonal IgG reagent from Antibodies.com detects protein pelota homolog (PELO), the mammalian ortholog of yeast Dom34 that mediates ribosome rescue and mRNA surveillance, including the no-go decay response to stalled translation complexes. The 200 µl vial is supplied for Western blot detection in human, mouse, and rat samples, and the unconjugated format supports flexible pairing with HRP- or fluorophore-conjugated secondary antibodies for chemiluminescent or fluorescent readouts. PELO accumulates on collided ribosomes during cellular stress, where it is investigated in the context of translation quality control, ribosome stalling, and integrated stress responses, and is also relevant to studies of neurodegeneration, hematopoiesis, and translational readthrough. The antibody targets conserved epitopes within the pelota factor and detects a band near the predicted molecular weight of approximately 43 kDa on standard SDS-PAGE gels. Aliquot upon arrival and store frozen at -20 °C, protecting the vial from repeated freeze-thaw cycles to preserve binding activity across long-term experiments. Intended for research use only.