Anti-PEF1 Antibody

Penta-EF-hand domain-containing protein 1 (PEF1) is a calcium-binding member of the EF-hand protein family, structurally related to calpain small subunits and ALG-2, with documented roles in calcium homeostasis, endoplasmic reticulum stress signaling, and the regulation of apoptotic cell death.

For researchers probing these pathways, the Antibodies.com anti-PEF1 reagent is a rabbit polyclonal IgG raised against the human protein and validated for Western blot detection. The unconjugated format, supplied in a 200 µL volume, allows flexible pairing with HRP, biotin, or fluorescent secondary antibodies depending on the chemiluminescent or near-infrared imaging platform in use.

The polyclonal composition provides broad epitope coverage, an advantage when target abundance is modest or when PEF1 migrates with subtle post-translational modifications that could mask a single epitope. Reactivity has been characterized against human samples, supporting immunoblotting work on whole-cell lysates derived from human cell lines and primary cultures.

Standard workflows involve SDS-PAGE separation of lysates prepared under reducing conditions, transfer to nitrocellulose or PVDF membranes, blocking, overnight primary incubation, and detection with a rabbit-specific secondary. Refrigerated storage preserves antibody activity across multiple blotting runs.