Anti-PECAM1 Antibody

Rabbit polyclonal IgG primary antibody against PECAM1 (also called CD31), supplied as 200 µl of unconjugated liquid reagent and validated for western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows on human, mouse, and rat specimens. The target is a ~130 kDa transmembrane glycoprotein expressed at vascular endothelium, platelets, and selected leukocyte populations, with broad epitope coverage useful when probing PECAM1 splice variants.

Across vascular biology, tumor angiogenesis, and cardiovascular research labs, this Antibodies.com reagent is used to map capillary networks and quantify microvessel density in tumor xenograft and wound-healing tissue sections. Immunofluorescence on HUVEC monolayers highlights the expected junctional pattern at cell-cell borders, while western blots on endothelial cell lysates resolve the antigen near 130 kDa. In immunoprecipitation the antibody pulls down endogenous PECAM1 for downstream interaction studies, including work on PECAM1 cytoplasmic-domain tyrosine phosphorylation events implicated in leukocyte transendothelial migration.

Storage recommendation: aliquot on arrival and store at -20 °C; the working vial should be kept on ice and protected from repeated freeze-thaw. For IHC on paraffin sections, heat-induced antigen retrieval in citrate buffer (pH 6.0) precedes overnight primary incubation. Provided by Antibodies.com as part of its Primary Antibodies catalog, the reagent integrates into standard immunolabeling pipelines for vascular, inflammation, and atherosclerosis studies.