Anti-PDLIM5 Antibody - Identical to Abcam (ab196559)

PDLIM5 (also known as Enigma Homolog, ENH) is a cytoplasmic scaffold combining an N-terminal PDZ domain with C-terminal LIM domains, anchoring the actin cytoskeleton to signaling complexes in striated muscle and neuronal tissue. This rabbit polyclonal IgG reagent from Antibodies.com is raised against a recombinant fragment of human PDLIM5 and affinity-purified for batch consistency, supplied unconjugated in a 50 µl liquid vial with PBS pH 7.4, 50% glycerol, and 0.02% sodium azide as a storage buffer.

Validated applications include Western blot at 1/500 to 1/2000 dilution for endogenous detection in cell and tissue lysates, and ICC/IF at 1/50 to 1/200 dilution for subcellular localization studies. Reactivity is tested and guaranteed on Human and Mouse samples, with Rat reactivity predicted from sequence homology. The reagent is identical to the well-characterized Abcam antibody catalogue ab196559, cited in peer-reviewed studies of cardiac and skeletal muscle differentiation, neuronal calcium signaling, and prostate cancer tumorigenesis.

For handling, aliquot immediately upon arrival and store at -20°C; avoid repeated freeze-thaw cycles. Working aliquots can be kept at +4°C for short-term use (1 to 2 weeks). The product ships on blue ice and is intended for research use only in western blot and immunofluorescence workflows.