Anti-PDLIM5 Antibody - Identical to Abcam (ab196559)

Raised in rabbit against a recombinant fragment of human PDLIM5 (UniProt Q96HC4), this affinity-purified polyclonal antibody is supplied as an unconjugated IgG in a 100 µL vial in liquid format. Distributed by Antibodies.com, the reagent is validated for western blot at a working dilution range of 1/500 to 1/2000 and for immunocytochemistry/immunofluorescence at 1/50 to 1/200, with published immunofluorescence images on U-2 OS cells at a 1/50 dilution. Reactivity has been confirmed on human and mouse samples, with rat reactivity predicted by homology. The PDLIM5 target, also known as Enigma homolog (ENH), is a LIM- and PDZ-domain scaffolding protein that localizes to actin stress fibers and intercalated discs, anchoring signaling components at sites of cell-cell contact in cardiac and neuronal tissue. Storage at –20 °C in PBS containing 50% glycerol and 0.02% sodium azide preserves activity through repeated freeze-thaw cycles, while the unconjugated format leaves choice of label, species cross-adsorption, and detection chemistry open to the researcher.