Anti-PDK4 Antibody

PDK4 (Pyruvate Dehydrogenase Kinase 4) is a mitochondrial serine/threonine kinase that phosphorylates the E1α subunit of the pyruvate dehydrogenase complex, suppressing glucose oxidation when fatty acids are the preferred fuel. This rabbit polyclonal reagent from Antibodies.com targets PDK4 in human and mouse samples and is supplied as 100 µL of unconjugated antiserum, ready for direct labeling or use with secondary detection systems in western blot and immunohistochemistry workflows.

Researchers studying metabolic switching, mitochondrial bioenergetics, and the fasting response use this antibody to track PDK4 upregulation in skeletal muscle, heart, and liver, where the kinase is induced by PPARα, FOXO1, and PGC-1α signaling. In oncology, PDK4 has attracted interest as a metabolic checkpoint in cancers that rely on glycolysis and as a contributor to drug resistance under hypoxic conditions. Cardiomyocyte studies leverage PDK4 expression to model the shift from carbohydrate to fatty acid oxidation in heart failure and diabetic cardiomyopathy.

For western blot, the antibody detects the endogenous ~46 kDa PDK4 band in tissue lysates from human and mouse origins. In immunohistochemistry, it produces cytoplasmic and mitochondrial staining patterns consistent with the kinase's submitochondrial localization. The polyclonal format provides multiple epitope recognition, which is useful when probing across species or when a single monoclonal epitope is occluded by post-translational modification or fixative-induced masking.

Storage recommendations and buffer composition are provided on the vial and accompanying datasheet from Antibodies.com. The unconjugated format allows flexibility with HRP-, biotin-, or fluorophore-conjugated secondaries chosen for a particular detection platform.