Anti-PDHX Antibody

This rabbit polyclonal antibody targets PDHX, the E3-binding protein subunit of the mitochondrial pyruvate dehydrogenase complex that links glycolysis to the citric acid cycle. Raised in rabbit and affinity-purified, the reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a stabilizing cryoprotectant. The unconjugated IgG is validated for Western blot and immunohistochemistry workflows on human tissue and cell samples, and it recognizes the native mitochondrial component responsible for tethering the E3 (dihydrolipoamide dehydrogenase) dimer to the E2 catalytic core.

PDHX is essential for pyruvate dehydrogenase complex assembly and function; loss-of-function variants in the gene underlie a form of pyruvate dehydrogenase deficiency that can present with neonatal lactic acidosis and neurodevelopmental involvement, making reliable detection in research samples valuable for studies of mitochondrial metabolism. The 100 µl vial format supports multiple blotting and staining experiments, and the polyclonal format provides tolerance for minor conformational variation in the target epitope. Store aliquots frozen at -20 °C and limit freeze-thaw cycles to preserve binding activity. This Antibodies.com reagent is intended for research use only and is not cleared for diagnostic or therapeutic procedures.