Anti-PDHA1 Antibody

PDHA1 (Pyruvate Dehydrogenase E1 Subunit Alpha 1) is the catalytic core of the pyruvate dehydrogenase complex, the mitochondrial gatekeeper that decarboxylates pyruvate to acetyl-CoA and channels carbon from glycolysis into the citric acid cycle. This rabbit polyclonal antibody from Antibodies.com is raised against the human PDHA1 protein and is validated for Western blot and immunohistochemistry workflows on human samples, with unconjugated format suitable for downstream labelling with HRP, biotin, or fluorophore-conjugated secondary reagents.

Supplied as 100 µl of polyclonal antiserum, the reagent recognizes native and denatured E1-alpha subunits in whole-cell lysates, mitochondrial fractions, and formalin-fixed tissue sections, supporting studies of oxidative phosphorylation, X-linked pyruvate dehydrogenase deficiency, and the metabolic reprogramming observed in cancer cells. The polyclonal backbone broadens epitope coverage across PDHA1, helping preserve signal across isoforms and post-translational modifications such as phosphorylation at Ser232, Ser293, and Ser300.

Store at -20 °C in working aliquots to preserve activity across multiple freeze-thaw cycles; thaw on ice and limit cycling of the stock vial. For Western blot, run 20-40 µg of total protein on SDS-PAGE and detect with an anti-rabbit IgG secondary; for IHC, perform antigen retrieval in citrate buffer (pH 6.0) before overnight incubation. Intended for research use only.