Anti-PDHA1 Antibody

This polyclonal antibody targets PDHA1 (Pyruvate Dehydrogenase E1 Subunit Alpha 1), the catalytic α-subunit of the mitochondrial pyruvate dehydrogenase complex that converts pyruvate to acetyl-CoA and links glycolysis to the tricarboxylic acid cycle. Raised in rabbit and supplied unconjugated in a 100 µl volume, the reagent is validated for Western blot, immunohistochemistry, and ELISA workflows, with reactivity confirmed against Human, Mouse, and Rat samples.

Investigators studying mitochondrial bioenergetics, the Warburg effect in tumour metabolism, X-linked pyruvate dehydrogenase deficiency, and Leigh syndrome use this Primary Antibody from Antibodies.com to probe PDH complex assembly, enzymatic regulation by phosphorylation at Ser293, and metabolic switching in cell culture lysates, frozen tissue sections, and recombinant protein preparations. The unconjugated format allows flexible pairing with custom fluorophore or horseradish peroxidase secondary reagents for immunofluorescence, chromogenic detection, and quantitative immunoblotting experiments.