Anti-PDGFR-α Antibody

Manufactured for research laboratories by Antibodies.com, this affinity-purified reagent is a polyclonal antibody raised in rabbit against the platelet-derived growth factor receptor alpha (PDGFR-α) protein. Supplied as 50 µl of an unconjugated IgG preparation at a concentration of 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, it is validated for Western blot (WB) and immunohistochemistry (IHC) workflows and shows reactivity with Human, Mouse and Rat samples. The antibody was raised against a synthesized peptide derived from the internal region of human PDGFR-α, then purified from rabbit antiserum by affinity chromatography using the epitope-specific immunogen, allowing it to detect endogenous levels of total PDGFR-α protein in target lysates and tissue sections. Recommended applications include western blotting to confirm receptor expression in cell or tissue extracts and IHC to localize PDGFR-α staining in fixed sections, making the product a versatile tool for studies of receptor tyrosine kinase signalling, fibroblast biology and tumour-stroma interactions. For storage stability, the buffered glycerol formulation should be kept at –20 °C in single-use aliquots to avoid repeated freeze–thaw cycles, with sodium azide included as a preservative. This format from Antibodies.com is intended for research use only and is not approved for diagnostic or therapeutic applications.