Anti-PDE5A Antibody

The Antibodies.com Anti-PDE5A polyclonal reagent is a rabbit-hosted primary antibody raised against phosphodiesterase 5A, the cGMP-hydrolyzing enzyme implicated in smooth muscle relaxation, vascular tone, and erectile function research. Supplied as 100 µl of unconjugated antiserum, the antibody is validated for Western blot and immunohistochemistry workflows on human tissue sections and lysates.

Investigators studying NO–cGMP signaling, pulmonary hypertension models, or corpus cavernosum tissue typically use this reagent to probe the canonical ~100 kDa PDE5A band in WB and to localize expression in IHC preparations. The unconjugated format leaves the choice of HRP, biotin, or fluorophore-conjugated secondary antibody to the end user, supporting flexible chromogenic, immunofluorescence, and immunoperoxidase detection setups. Polyclonal reactivity against multiple epitopes of human PDE5A improves tolerance for minor sample variation between donor specimens and across fixation conditions used in IHC.

Storage recommendation: aliquot upon receipt and store refrigerated for short-term use or freeze for longer-term stability, protecting the vial from repeated freeze-thaw cycles to preserve binding activity.