Anti-PDE4D Antibody

PDE4D (Phosphodiesterase 4D) is a key regulator of intracellular cyclic AMP signaling, hydrolyzing cAMP to modulate downstream pathways involved in inflammation, cognition, and smooth muscle contractility. This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit and affinity-purified to recognize PDE4D across multiple isoforms, supporting tracking of cAMP-pathway activity in disease models of asthma, COPD, and neurodegeneration.

The antibody is supplied as 100 µl of liquid at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, a cryoprotective formulation that preserves activity during long-term storage at -20 °C with minimal freeze-thaw cycling. Researchers aliquot on first use to avoid repeated cycles. Validated applications include Western blot for detecting endogenous PDE4D in cell lysates from human, mouse, and rat samples, and immunohistochemistry on fixed tissue sections to localize PDE4D distribution in brain, lung, and inflammatory infiltrates.

Cross-reactivity testing demonstrates recognition of PDE4D isoforms across human, mouse, and rat samples, enabling translational studies that bridge rodent models and patient-derived material. The unconjugated format gives researchers flexibility to pair the primary with HRP, biotin, or fluorescent secondary reagents of their preference, adapting the assay to chemiluminescent or fluorescence-based readout platforms.

This vial-format reagent fits target-validation work in PDE4 inhibitor discovery programs (e.g., studies of roflumilast and analogous compounds) and into basic research on cAMP-mediated processes in neuroscience and immunology, where PDE4D modulation remains a focal therapeutic hypothesis. For research use only.