Anti-PDCD10 Antibody

Raised in rabbit and affinity-purified to an IgG backbone, this polyclonal reagent detects endogenous PDCD10 (also called CCM3), a scaffold protein best characterized for its role in cerebral cavernous malformation signaling and endothelial cell biology. The unconjugated antibody is supplied at 100 µl per vial, so laboratories can pair it with an HRP- or biotin-based secondary system of their own choosing in immunohistochemistry workflows on formalin-fixed paraffin-embedded tissue.

Validation work distributed by Antibodies.com confirms reactivity on human and rat sections, making the reagent a practical tool for cross-species studies of vascular development, apoptosis regulation, and Rho-GTPase signaling downstream of the CCM complex. End users typically apply dilutions in the 1:100 to 1:500 range on 4 µm paraffin sections after citrate-buffer antigen retrieval, with signal developed at the DAB chromogen step.

For best lot-to-lot consistency, store the vial at -20 °C in working aliquots and avoid repeated freeze-thaw cycles. Each shipment from Antibodies.com leaves on cold pack to preserve antibody activity on arrival, and the unconjugated format keeps the reagent compatible with immunofluorescence, immunoprecipitation, or Western blot protocols when a project needs to extend beyond IHC. Researchers investigating CCM3 biology, vascular malformation pathology, and programmed cell-death pathways will find this polyclonal useful as a primary detection reagent.