Anti-PCNA Antibody

This unconjugated rabbit polyclonal reagent targets PCNA, the homotrimeric DNA sliding clamp that encircles replicating chromatin and acts as a processivity factor for DNA polymerase delta. Because PCNA expression rises sharply during S-phase, antibodies against it are routinely used to score actively dividing cells in tissue sections and whole-cell lysates. Antibodies.com supplies this reagent as a 100 µl liquid, unconjugated and affinity-purified, optimized for Western blot and immunohistochemistry workflows. It cross-reacts with Human, Mouse, and Rat samples, enabling comparative work between patient biopsies and rodent models of liver regeneration, tumor xenografts, and neural stem cell expansion. In immunohistochemistry the reagent produces crisp nuclear staining that pairs with Ki-67 or BrdU for cell-cycle-position analysis, while in Western blot it yields a discrete band near the expected molecular weight of endogenous PCNA. Aliquot on arrival, store refrigerated in working volumes, and protect from light to preserve binding activity through months of repeated use.