Anti-PCNA Antibody

This mouse monoclonal IgG1 antibody from Antibodies.com targets Proliferating Cell Nuclear Antigen (PCNA), a 36 kDa homotrimeric nuclear protein that acts as a sliding clamp for DNA polymerase delta during replication. It is widely used as an endogenous marker of actively dividing cells in oncology, developmental biology, and tissue regeneration studies.

Validated across multiple assay formats, the reagent supports Western blot analysis of whole-cell lysates, immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, and flow cytometry for profiling proliferating populations. It cross-reacts with Human, Mouse, and Rat samples, making it useful for comparative work in cultured lines and primary tissue from each species.

Each vial contains 100 µL of unconjugated antibody formulated in TBS buffer at pH 7.4, supplemented with 1% BSA and 40% glycerol as stabilizers, and 0.05% sodium azide as preservative. The product is supplied as a ready-to-dilute liquid; aliquot upon first use and store at 4°C to preserve activity. Avoid repeated freeze-thaw cycles, and protect from light when handling open vials.

In Western blot, the antibody typically yields a clean band near the expected molecular weight, while in IHC it labels nuclei of proliferating cells in germinal centers, intestinal crypts, and developing tissues. Researchers studying DNA replication, cell cycle regulation, and tumor growth rely on PCNA detection to distinguish dividing from quiescent populations.