Anti-PCNA Antibody

Offered by Antibodies.com as a primary antibody, this mouse monoclonal reagent targets Proliferating Cell Nuclear Antigen (PCNA), the conserved ~36 kDa nuclear protein that acts as a cofactor for DNA polymerase δ during DNA replication and is widely used as a marker of proliferating cells. The clone is supplied as a purified IgG in 50 µl of a Tris-Glycine buffer (pH 7.4) containing 150 mM NaCl, 0.2% sodium azide and 50% glycerol, and is unconjugated, making it suitable for use with secondary reagents of the user's choice. It is validated for Western blot (WB), immunoprecipitation (IP) and immunocytochemistry (ICC/IF) applications. The antibody shows reactivity with Human, Mouse, Rat, Monkey and Hamster samples, reflecting the strong evolutionary conservation of PCNA across mammalian species. Recommended long-term storage is at -20 °C in small aliquots to avoid repeated freeze/thaw cycles; for short-term use, the antibody is stable at +4 °C. This lot is supplied through AFMS for researchers needing a reliable, validated PCNA reagent for cell-cycle, DNA replication and proliferation studies.